ThreatLocker, the Orlando-based Zero Trust cybersecurity platform, said Tuesday it has raised $190 million in Series F funding to expand into the AI-agent era. Growth investor Elephant led the round, with continued backing from D. E. Shaw Ventures and Arthur Ventures and a new commitment from Koch Disruptive Technologies. The company plans to use the capital for AI-related security controls, broader platform development and international expansion beginning with a UK office.
Deny-by-default cybersecurity as AI agents scale
ThreatLocker sells prevention rather than detection, blocking any application, script or process that has not been explicitly allowlisted. The company says its Zero Trust platform already protects more than 70,000 organisations worldwide across endpoints, networks, cloud systems and storage, including JetBlue, Heathrow, the US Navy, University of Central Florida, Indianapolis Colts and Emirates Flight Catering.
The AI-Zero Trust nexus
The financing lands at a moment when enterprise attack surfaces are getting more fluid because AI agents and AI-generated code are now writing to production systems. Gartner's own May 2026 note "Shadow AI Demands Stricter Endpoint Application Control" argued that unmanaged AI runtime is one of the fastest-growing endpoint risks. That is the wedge ThreatLocker is selling for its next chapter: policy enforcement over what software, agents and users are allowed to do.
A crowded but well-funded cyber category
ThreatLocker's Series F is the largest cybersecurity round disclosed this week, dwarfing Microsoft's MAI-Cyber-1-Flash announcement, Intrusion's VigilAigent acquisition and AegisAI's $36M Series A. The company's Cyber Hero team, based in Orlando and available 24/7, is one of the few enterprise-support operations promising sub-60-second response times.
Reporting based on coverage from ThreatLocker, PR Newswire and Tech Startups.